Comparative study of different methods of microscopy followed by a culture method in the detection of mycobacterium tuberculosis in clinically and radiologically suspected cases of pulmonary tuberculosis

To compare the sensitivity of 2 microscopic methods for diagnosis Mycobacterium tuberculosis (M.tb) along with culture and drug susceptibility testing to first line drugs. : The cross-sectional study comprises 200 suspected cases pulmonary both clinically radiologically in KIMS, Bangalore over a period years. Samples (sputum/BAL fluid) were collected, processed stained by Ziehl Neelson (ZN) Fluorescent methods. Culture susceptibilty was done Streptomycin, Isoniazid, Rifampicin Ethambutol growth indicator tube (MGIT) method after decontamination. Fischer’s test 1. Out samples: 1.120 male 80 female; 2. 18 positive Neelson, 21 28 culture; 3. Majority patients belonged age group 41-50 years (23%); 4. InMGIT, 26 M.tb Non-tubercular mycobacteria; 5. isolates, 4 resistant streptomycin, 6 isoniazid, rifampicin 9 ethambutol. staining (64.28%) is higher than that Ziehl-Neelson (51.7%); In MGIT, Non tubercular Multi-drug resistant- (MDR-TB) This made us aware need prompt detection, identification appropriate treatment Tuberculosis due rising incidence MDR-TB.